The Gadsden flag, proposing a rattlesnake and the word "Don't Tread on Me

https://miloowdz632.oakmontscope.com/posts/the-significance-of-colors-in-civil-war-flags

The Gadsden flag, providing a rattlesnake and the word "Don't Tread on Me," used to be adopted via a few Confederate units as a symbol of resistance towards perceived oppression.