The Gadsden flag, presenting a rattlesnake and the word "Don't Tread on Me

https://edwinttoq839.evergrovio.com/posts/historic-civil-war-flags-understanding-their-role-within-the-conflict

The Gadsden flag, offering a rattlesnake and the word "Don't Tread on Me," used to be followed by a few Confederate devices as a symbol of resistance towards perceived oppression.